Culture of Human Keratinocytes in Liquid Medium
نویسندگان
چکیده
منابع مشابه
In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold
Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...
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BACKGROUND Traditionally, the Lowenstein Jensen (LJ) medium has been used for culturing Mycobacterium tuberculosis. In abdominal tuberculosis (TB), the reported yield from tissue culture is between 20% and 60%. Liquid cultures are reported to give a higher yield but there is little data available in abdominal TB. AIM To compare the yield of TB culture with BACTEC 460TB liquid medium and LJ me...
متن کاملAbsence of tumorigenicity in athymic mice by normal human epidermal keratinocytes after culture in serum-free medium.
The very rapid growth rate (1 population doubling/day) of normal human epidermal keratinocytes (HK) cultured in serum-free medium can be utilized for wound closure in burn treatment. However, rapid growth in vitro may present the possibility of neoplastic transformation. To investigate this possibility, HK were cultured from primary isolation to large populations in MCDB 153 medium supplemented...
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متن کاملin vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold
background: extensive full-thickness burns require replacement of both epidermis and dermis. in designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. methods: in the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1983
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12540284